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41.
42.
(Quasi‐)Racemic X‐ray Structures of Glycosylated and Non‐Glycosylated Forms of the Chemokine Ser‐CCL1 Prepared by Total Chemical Synthesis 下载免费PDF全文
Prof. Dr. Ryo Okamoto Dr. Kalyaneswar Mandal Dr. Michael R. Sawaya Prof. Dr. Yasuhiro Kajihara Prof. Dr. Todd O. Yeates Prof. Dr. Stephen B. H. Kent 《Angewandte Chemie (International ed. in English)》2014,53(20):5194-5198
Our goal was to obtain the X‐ray crystal structure of the glycosylated chemokine Ser‐CCL1. Glycoproteins can be hard to crystallize because of the heterogeneity of the oligosaccharide (glycan) moiety. We used glycosylated Ser‐CCL1 that had been prepared by total chemical synthesis as a homogeneous compound containing an N‐linked asialo biantennary nonasaccharide glycan moiety of defined covalent structure. Facile crystal formation occurred from a quasi‐racemic mixture consisting of glycosylated L ‐protein and non‐glycosylated‐D ‐protein, while no crystals were obtained from the glycosylated L ‐protein alone. The structure was solved at a resolution of 2.6–2.1 Å. However, the glycan moiety was disordered: only the N‐linked GlcNAc sugar was well‐defined in the electron density map. A racemic mixture of the protein enantiomers L ‐Ser‐CCL1 and D ‐Ser‐CCL1 was also crystallized, and the structure of the true racemate was solved at a resolution of 2.7–2.15 Å. Superimposition of the structures of the protein moieties of L ‐Ser‐CCL1 and glycosylated‐L ‐Ser‐CCL1 revealed there was no significant alteration of the protein structure by N‐glycosylation. 相似文献
43.
A low-cost optical feedback system using dynamic mirrors has been developed at the NSLS for stabilizing the position and direction of an infrared synchrotron beam against thermal drift and mechanical noise. The system design has some unique features that potentially simplify installation into an existing infrared beamline. We describe the system and its features along with some performance results. 相似文献
44.
We propose and experimentally demonstrate a system for the generation of pulses of tunable pulse-width as those required in high spectral efficiency optically routed networks. Pulse narrowing of 500 ps pulses by 90% is accomplished through a SOA based non-linear loop mirror. Optical switching through the SOA loop mirror is used to shape and carve these large pulses (e.g., 500 ps) generated by non-expensive low-frequency optoelectronic components to narrow pulses (e.g., 50 ps). We also calculate the minimum loop size and optimum repetition rate of the original pulse train for the generation of the shorter pulse-width pulse train. 相似文献
45.
Joonmo Kim Mihaela Cardei Ionut Cardei Xiaohua Jia 《Journal of Global Optimization》2002,24(4):437-448
In this paper, we present the design of a Polynomial Time Approximation Scheme (PTAS) for the Grade of Service Steiner Minimum Tree (GOSST) problem, which is known to be NP-Complete. Previous research has focused on geometric analyses and different approximation algorithms have been designed. We propose a PTAS that provides a polynomial time, near-optimal solution with performance ratio 1+. The GOSST problem has some important applications. In network design, a fundamental issue for the physical construction of a network structure is the interconnection of many communication sites with the best choice of the connecting lines and the best allocation of the transmission capacities over these lines. Good solutions should provide paths with enough communication capacities between any two sites, with the least network construction costs. Also, the GOSST problem has applications in transportation, for road constructions and some potential uses in CAD in terms of interconnecting the elements on a plane to provide enough flux between any two elements. 相似文献
46.
高速扫描相机时间测量不确定度分析 总被引:21,自引:6,他引:15
以国内普遍使用的SJZ-30型高速扫描相机为例,用精测转速方法的结果处理数据,转速测量相对合成不确定度小于0.1%,时间间隔测量的相对扩展不确定度为0.2%.扫描速度在像面上的位置误差,采用"中值扫描速度",可予以校正.同时讨论了进一步降低转速测量不确定度和位置误差的方法. 相似文献
47.
E. Lazzaro R. Coelho 《The European Physical Journal D - Atomic, Molecular, Optical and Plasma Physics》2002,19(1):97-101
A major limit to steady state and advanced high operation of tokamaks of reactor class is due to the onset of tearing modes that develop magnetic and may cause loss of energy confinement or a major disruption. Here the structure of a classical problem
about the effects of external control helical fields is analysed and it is shown to offer a general paradigm of response of
low order classical and neoclassical tearing modes to a wide class of external perturbations. New results of principle on
the structural stability of the response model are obtained, leading to a clear interpretation of the role of “seed islands"
in the onset of neo-classical tearing modes and the role of finite ion larmor radius corrections to Ohm's law.
Received 12 November 2001 and Received in final form 4 January 2002 相似文献
48.
49.
分析了制作高功率连续激光反射镜材料的热性能、缺陷及其加工工艺, 提出了制作高功率激光反射镜应考虑的几个关键问题: 反射镜材料的综合热性能比值S, 材料的微观结构、缺陷的大小和晶向的选择, 以及加工工艺的设计。并介绍了实验结果。 相似文献
50.
研究了一种新型光纤环镜(FLM)的原理与特性,这种FLM由在普通光纤环镜中插入光纤型偏振控制器(PC)构成.通过等效光路分析建立了该FLM的理论模型,并对其反射特性进行了数值模拟.研究表明,通过改变PC的状态,即改变其双折射效应的快轴取向或强度,可连续调节FLM的反射率,反射率谱具有宽带特性,主要受光纤耦合器工作带宽的影响.此外,对FLM的反射特性还进行了实验研究.实验结果也证实,通过调节PC状态,FLM反射率可在其最大和最小值之间连续调节,实验测得FLM最大和最小反射率分别可达93%和2%.根据PC双折
关键词:
光纤环镜
偏振控制器
光纤耦合器
反射率 相似文献